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  1. X

    --unal (0,1) question

    Oh, I found it, thanks really!
  2. X

    --unal (0,1) question

    For unaligned sequences, will it be reported in the BLAST tabular format? I tried to use the --unal 1 with -f 6, but it doesn't show the unaligned sequences.
  3. X

    Memory cost problem in makedb

    It works! Thanks, really! So, I don't really know what is going on with the WSL2 and Windows. I asked my friend and he told me it could be the resource distribution of a virtual machine. Also, the install .exe document issue could be caused by the existed Diamond program. I installed it on the...
  4. X

    Memory cost problem in makedb

    Does this mean I have already installed the diamond? But I only installed it on Linux, not sure how it works with WSL2. Do I need to delete it?
  5. X

    Memory cost problem in makedb

    I am using WSL2 on window with ubuntu. I tried native windows though, but there is no response after run anyways.
  6. X

    Memory cost problem in makedb

    Unfortunately, 32GB is not enough for running makedb with taxonomy. Is it possible to run it on a local computer instead of the server?
  7. X

    Memory cost problem in makedb

    Sure, thanks!
  8. X

    Memory cost problem in makedb

    I really appreciate the fast reply. In this case, cause I am going to build a new pc, is it necessary to build the memory with 64GB so that I can makedb with taxonomy, or 32GB is enough.
  9. X

    Memory cost problem in makedb

    I am trying to make database with taxonmap. Here is my code: sudo diamond makedb --in /mnt/e/blastdb/nr_database/nr --db nr --taxonmap /mnt/e/diamond/prot.accession2taxid --taxonnames /mnt/e/diamond/taxdmp/names.dmp --threads 4 --taxonnodes /mnt/e/diamond/taxdmp/nodes.dmp Is there any way...
  10. X

    Question about --taxonlist

    I just send you the data. Also, I checked the version, its probably too old, v0.9.25.126. Do I have any options to update the version on Linux without using bioconda? Or I just need to redownload.
  11. X

    Question about --taxonlist

    Thanks for the fast reply! This is the result without taxonomy filter, --taxonlist 2. This is the result of --masking 0 without --sensitive or --very-sensitive. When I try --sensitive the performance will be better. But the --very-sensitive will cause a dramatically decline of the reports...
  12. X

    Question about --taxonlist

    I was trying to do blastp for about 2000 protein sequences against nr database. Specifically, I want to blast with bacteria protei, so I used --taxonlist 2. However, it only returned about 900 results. To make sure what happened with the sequences which are not showed on the results, I tested 2...
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